Chinese Journal of Hospital Pharmacy

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Immunotherapeutic effect of dendritic cell vaccine assisted by Glycyrrhiza inflata polysaccharides in H22 hepatoma-bearing mice

YU Yongting, NADIREMU Xiaokelati, LU Yongqiang, ZHOU Zhaoxi, CONG Yuanyuan

Chinese Journal of Hospital Pharmacy, 2024, 44(1): 35-41. DOI: 10.13286/j.1001-5213.2024.01.06

分组	胸腺指数	脾脏指数	肝脏指数
模型组（n＝10）	1.04±0.20	6.82±1.00	51.85±5.15
阳性组（n＝7）	1.04±0.27	12.29±2.93^b	63.13±9.08^b
TNF-α组（n＝10）	1.58±0.61^a	9.36±2.79^a	55.30±5.87
DC组（n＝10）	1.56±0.41^a	10.85±2.17^b	58.91±7.98^a
GiP组（n＝10）	1.88±0.67^b	9.60±2.10^b	62.35±9.46^b
GiP-B1组（n＝10）	2.02±0.51^b	8.59±2.33	55.86±6.33

Tab 3 Effect of immunotherapy on the organ index of tumor-bearing mice （mg·g^–1，$\bar{x}±s$）

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Fig 1 Morphological changes of BMDC during culture （×200）
Fig 2 Form of DC vaccine in each group （×200）
Tab 1 Expression of the surface marker molecules of DC vaccine in each group （%，n＝3，$\bar{x}±s$）
Fig 3 Expression of the surface marker molecules of Glycyrrhiza uralensis polysaccharide-assisted DC vaccine
Fig 4 Changes of the body mass of tumor-bearing mice in each group during immunotherapy 注（note）：与模型组相比（vs. model group），^aP<0.05，^bP<0.01。
Tab 2 Effects of immunotherapy on tumor weight， tumor volume and tumor inhibition rate in tumor-bearing mice （$\bar{x}±s$）
Fig 5 The tumor photos of tumor-bearing mice in each group during inmunotherapy
Tab 4 Effect of immunotherapy on the content of cytokines in tumor-bearing mice （$\bar{x}±s$）
Fig 6 Effect of Glycyrrhiza inflata polysaccharide-assisted DC vaccine on liver tissue of tumor-bearing mice （×200）
Fig 7 Effect of Glycyrrhiza uralensis polysaccharide-assisted DC vaccine on tumor tissue of tumor-bearing mice （×200）
Fig 8 Effect of Glycyrrhiza inflata polysaccharide-assisted DC vaccine on the expression of Bax in tumor tissue of tumor-bearing mice （×400）
Fig 9 Effect of Glycyrrhiza inflata polysaccharide-assisted DC vaccine on the expression of Bcl-2 in tumor tissue of tumor-bearing mice （×400）
Tab 5 Effect of DC vaccine assisted by Glycyrrhiza inflata polysaccharide on the expression of Bax and Bcl-2 protein in tumor tissues（$\bar{x}±s$）

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